Proteomic analysis of human follicular fluid associated with successful in vitro fertilization
نویسندگان
چکیده
منابع مشابه
Proteomic analysis of human follicular fluid associated with successful in vitro fertilization
BACKGROUND Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome. METHODS To better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/io...
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Background: Induction of in vitro maturation and development of immature oocyte is one of the methods that has been frequently used recently in artificial reproduction techniques and has drawn attention of many investigators. This method is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment ...
متن کاملFatty Acid Composition of Human Follicular Fluid Phospholipids and Fertilization Rate in Assisted Reproductive Techniques
Background: Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women’s fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid ph...
متن کاملThe Effect of Human Follicular Fluid (FF) on Preimplantation Mouse Embryo Development in Vitro
SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...
متن کاملN-glycoproteomic analysis of human follicular fluid during natural and stimulated cycles in patients undergoing in vitro fertilization
OBJECTIVE Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human foll...
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ژورنال
عنوان ژورنال: Reproductive Biology and Endocrinology
سال: 2017
ISSN: 1477-7827
DOI: 10.1186/s12958-017-0277-y